Biotechnology : Principles and Processes
Subject matter experts of JagranJosh have developed chapter wise practice questions of Class 12th as per the relevancy of the chapter in the CBSE board exam. This practice set contains 10 questions with detailed explanations on “Chapter: Biotechnology : Principles and Processes” of Mathematics.
Q1.Which enzyme is used to cut a DNA fragment of interest by a molecular biologist?
Q2.What is the enzymatic function of restriction enzymes?
Q3.How does a bacteria defend its own DNA from restriction enzymes?
Q4.Which is the most accurate sequence of steps for a usual recombinant DNA and cloning?
I. Transform bacteria with recombinant DNA molecule.
II. Cut the plasmid DNA using restriction enzymes.
III. Extract plasmid DNA from bacterial cells.
IV. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.
V. Use ligase to seal plasmid DNA to nonplasmid DNA.
Q5.Why are yeast cells commonly used as hosts for cloning?
Q6.Yeast artificial chromosomes contain which of the following elements?
Q7.Arrange according to the correct cycle of PCR?
1. The primers hybridize to the target DNA.
2. The mixture is heated to a high temperature to denature the double stranded target DNA.
3. Fresh DNA polymerase is added.
4. DNA polymerase extends the primers to make a copy of the target DNA.